si bag2 Search Results


si bag2  (TargetMol)
93
TargetMol si bag2
Figure 2 Inhibition of BAG2 reduced keloid collagen synthesis and deposition. (A) The relative gene expression of COL1A1 and TIMP1 in <t>si-BAG2-treated</t> keloid fibroblasts compared to control groups. Gene expression related to collagen synthesis and the inhibition of collagen degradation was significantly reduced following BAG2 inhibition; (B) Western blot analysis showing reduced protein expression of BAG2, COL1, COL3, and α-SMA in si-BAG2-treated keloid fibroblasts; (C) Masson’s trichrome staining of keloid explants from different groups. Collagen structures in the si-BAG2-treated groups appeared thinner compared to control groups; (D) Western blot analysis demonstrates decreasing protein expression of COL1 and COL3 in si-BAG2-treated ex-vivo keloid explants. The reduction in COL1 and COL3 protein expression was statistically significant. Statistical analysis of relative protein expression is provided (n=3). **P<0.01, **** P<0.0001.
Si Bag2, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/si bag2/product/TargetMol
Average 93 stars, based on 1 article reviews
si bag2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
bag2 overexpression lysate denatured  (Novus Biologicals)
N/A
   Buy from Supplier
sgrna/cas9 all-in-one expression clones targeting bag2  (Genecopoeia)
N/A
Transfect cells with our CRISPR plasmids with Cas9 and sgRNA for human, mouse, and rat. Search our database of more than 45,000 human, mouse, and rat genes for genome editing using CRISPR. sgRNA expression plasmids
   Buy from Supplier
orf expression clone for bag2  (Genecopoeia)
N/A
GeneCopoeia offers the largest collection of ORF cDNA clones, with genome-wide coverage for human and mouse. All ORF cDNAs can be readily expressed in combination with a variety of fusion tags (fluorescence, antibody, solubility, purification
   Buy from Supplier
bag2 rat sirna oligo duplex  (OriGene)
N/A
Bag2 Rat 3 unique 27mer siRNA duplexes 2 nmol each
   Buy from Supplier
mirna 3´utr target expression clone for human bag2  (Genecopoeia)
N/A
GeneCopoeia offers genome-wide human, mouse and rat microRNA (miRNA) 3′ UTR target clones in mammalian expression vectors. miRNA 3′ UTR target clones can be used for miRNA target identification and functional validation of predicted targets,
   Buy from Supplier
human bag2 gene orf cdna clone expression plasmid c gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human BCL2 associated athanogene 2 with C terminal GFPSpark tag
   Buy from Supplier
bag2 nm 004282 human over expression lysate  (OriGene)
N/A
BAG2 HEK293T cell transient overexpression lysate as WB positive control
   Buy from Supplier
bag2 mouse sirna oligo duplex  (OriGene)
N/A
Bag2 Mouse 3 unique 27mer siRNA duplexes 2 nmol each
   Buy from Supplier
bag2 overexpression lysate  (Novus Biologicals)
N/A
BAG2 Overexpression Lysate
   Buy from Supplier
human bag2 gene orf cdna clone expression plasmid n myc tag  (Sino Biological)
N/A
Full length Clone DNA of Human BCL2 associated athanogene 2 with N terminal Myc tag
   Buy from Supplier

Image Search Results


Figure 2 Inhibition of BAG2 reduced keloid collagen synthesis and deposition. (A) The relative gene expression of COL1A1 and TIMP1 in si-BAG2-treated keloid fibroblasts compared to control groups. Gene expression related to collagen synthesis and the inhibition of collagen degradation was significantly reduced following BAG2 inhibition; (B) Western blot analysis showing reduced protein expression of BAG2, COL1, COL3, and α-SMA in si-BAG2-treated keloid fibroblasts; (C) Masson’s trichrome staining of keloid explants from different groups. Collagen structures in the si-BAG2-treated groups appeared thinner compared to control groups; (D) Western blot analysis demonstrates decreasing protein expression of COL1 and COL3 in si-BAG2-treated ex-vivo keloid explants. The reduction in COL1 and COL3 protein expression was statistically significant. Statistical analysis of relative protein expression is provided (n=3). **P<0.01, **** P<0.0001.

Journal: Biologics

Article Title: Therapeutic Potential of Compounds with High Affinity to BAG2 in Inhibiting Keloid Disease

doi: 10.2147/btt.s533286

Figure Lengend Snippet: Figure 2 Inhibition of BAG2 reduced keloid collagen synthesis and deposition. (A) The relative gene expression of COL1A1 and TIMP1 in si-BAG2-treated keloid fibroblasts compared to control groups. Gene expression related to collagen synthesis and the inhibition of collagen degradation was significantly reduced following BAG2 inhibition; (B) Western blot analysis showing reduced protein expression of BAG2, COL1, COL3, and α-SMA in si-BAG2-treated keloid fibroblasts; (C) Masson’s trichrome staining of keloid explants from different groups. Collagen structures in the si-BAG2-treated groups appeared thinner compared to control groups; (D) Western blot analysis demonstrates decreasing protein expression of COL1 and COL3 in si-BAG2-treated ex-vivo keloid explants. The reduction in COL1 and COL3 protein expression was statistically significant. Statistical analysis of relative protein expression is provided (n=3). **P<0.01, **** P<0.0001.

Article Snippet: Cell Cycle Analysis KFs were treated with si-BAG2, C16-PAF (Targetmol, #T21547, 10 nM), or si-BAG2 alone for 24 and 48 hours, along with the si-NC control.

Techniques: Inhibition, Gene Expression, Control, Western Blot, Expressing, Staining, Ex Vivo

Figure 3 Inhibition of BAG2 reduced KF migration and proliferation via the MEK signaling pathway. (A) Representative images and bar graph depict the migration of KFs treated with or without si-BAG2 at 0 and 24 hours after scratching (n=3). Scale bar = 500 μm; (B) CCK-8 assays were performed on control and si-BAG2-treated KFs, showing a significant reduction in the proliferation of si-BAG2-treated cells (n=3); (C) Relative gene expression of TGF-β in si-BAG2-treated and control KFs, with significantly decreased TGF-β expression observed following si-BAG2 treatment (n=3); (D) Western blot analysis revealing decreased p-MEK in si-BAG2-treated cells; (E) Representative cell cycle profiles of KFs from various groups, assessed by flow cytometry, accompanied by statistical analysis (n=3). *P<0.05, ** P<0.01, ***P<0.001, **** P<0.0001. KF, keloid fibroblast.

Journal: Biologics

Article Title: Therapeutic Potential of Compounds with High Affinity to BAG2 in Inhibiting Keloid Disease

doi: 10.2147/btt.s533286

Figure Lengend Snippet: Figure 3 Inhibition of BAG2 reduced KF migration and proliferation via the MEK signaling pathway. (A) Representative images and bar graph depict the migration of KFs treated with or without si-BAG2 at 0 and 24 hours after scratching (n=3). Scale bar = 500 μm; (B) CCK-8 assays were performed on control and si-BAG2-treated KFs, showing a significant reduction in the proliferation of si-BAG2-treated cells (n=3); (C) Relative gene expression of TGF-β in si-BAG2-treated and control KFs, with significantly decreased TGF-β expression observed following si-BAG2 treatment (n=3); (D) Western blot analysis revealing decreased p-MEK in si-BAG2-treated cells; (E) Representative cell cycle profiles of KFs from various groups, assessed by flow cytometry, accompanied by statistical analysis (n=3). *P<0.05, ** P<0.01, ***P<0.001, **** P<0.0001. KF, keloid fibroblast.

Article Snippet: Cell Cycle Analysis KFs were treated with si-BAG2, C16-PAF (Targetmol, #T21547, 10 nM), or si-BAG2 alone for 24 and 48 hours, along with the si-NC control.

Techniques: Inhibition, Migration, CCK-8 Assay, Control, Gene Expression, Expressing, Western Blot, Flow Cytometry